Composite

Part:BBa_K4399011:Design

Designed by: Duoqing LIN   Group: iGEM22_Worldshaper-Shanghai   (2022-09-27)


P2x35S-XVE-T35S


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1643
    Illegal PstI site found at 1827
    Illegal PstI site found at 1998
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1827
    Illegal PstI site found at 1998
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 222
    Illegal BglII site found at 549
    Illegal BglII site found at 1777
    Illegal BamHI site found at 1346
    Illegal XhoI site found at 753
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1643
    Illegal PstI site found at 1827
    Illegal PstI site found at 1998
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1643
    Illegal PstI site found at 1827
    Illegal PstI site found at 1998
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Transcription of XVE (BBa_K4399014) is initiated by P2×35S (BBa_K4399001) and terminated by T35s (BBa_K4399007).


Source

The sequence is based on BBa_K4399001, BBa_K4399014 and BBa_K4399007.


References