Composite
Part:BBa_K4399011:Design
Designed by: Duoqing LIN Group: iGEM22_Worldshaper-Shanghai (2022-09-27)
P2x35S-XVE-T35S
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1643
Illegal PstI site found at 1827
Illegal PstI site found at 1998 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1827
Illegal PstI site found at 1998 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 222
Illegal BglII site found at 549
Illegal BglII site found at 1777
Illegal BamHI site found at 1346
Illegal XhoI site found at 753 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1643
Illegal PstI site found at 1827
Illegal PstI site found at 1998 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1643
Illegal PstI site found at 1827
Illegal PstI site found at 1998 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Transcription of XVE (BBa_K4399014) is initiated by P2×35S (BBa_K4399001) and terminated by T35s (BBa_K4399007).
Source
The sequence is based on BBa_K4399001, BBa_K4399014 and BBa_K4399007.